Cold Spring Harbor Laboratory
Mouse Development, Stem Cells, and Cancer Course
June 6-25, 2018
Cold Spring Harbor, NY
Application deadline: March 15, 2018
This intensive lecture and laboratory course is designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.
The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells and fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, flow cytometry and hematopoietic stem cell transplantation, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.
Confirmed Speakers for 2018:
Kathryn Anderson, Memorial Sloan Kettering Cancer Institute
Richard Behringer, MD Anderson Cancer Center
Blanche Capel, Duke University
Frédéric Charon, Montreal Clinical Research Institute
Anna-Katerina Hadjantonakis, Memorial Sloan Kettering Cancer Center
Brigid Hogan, Duke University School of Medicine
Diana Laird, University of California San Francisco Medical Center
Jesse Mager, University of Massachusetts, Amherst
Francesca Mariani, University of Southern California
Andrew McMahon, University of Southern California
Olivier Pourquié, Harvard Medical School
Jason Rock, Boston University
Patrick Stover, Cornell University
Paul Trainor, Stowers Institute for Medical Research
Roll of honor: these are our alumni from 1983-2017:
Course participants - typically a mix of graduate students, postdoctoral fellows, research professionals and faculty members - are selected on a competitive basis for the 14 available slots. Stipends available.